[HTML][HTML] The generation of the oxidized form of creatine kinase is a negative regulation on muscle creatine kinase
Muscle creatine kinase (CK) is a crucial enzyme in energy metabolism, and it exists in two
forms, the reduced form (R-CK) and the oxidized form (O-CK). In contrast with R-CK, O-CK
contained an intrachain disulfide bond in each subunit. Here we explored the properties of O-
CK and its regulatory role on muscle CK. The intrachain disulfide bond in O-CK was
demonstrated to be formed between Cys 74 and Cys 146 by site-directed mutagenesis.
Biophysical analysis indicated that O-CK showed decreased catalytic activity and that it …
forms, the reduced form (R-CK) and the oxidized form (O-CK). In contrast with R-CK, O-CK
contained an intrachain disulfide bond in each subunit. Here we explored the properties of O-
CK and its regulatory role on muscle CK. The intrachain disulfide bond in O-CK was
demonstrated to be formed between Cys 74 and Cys 146 by site-directed mutagenesis.
Biophysical analysis indicated that O-CK showed decreased catalytic activity and that it …