[HTML][HTML] G-protein-coupled receptor kinase specificity for β-arrestin recruitment to the β2-adrenergic receptor revealed by fluorescence resonance energy transfer
JD Violin, XR Ren, RJ Lefkowitz - Journal of biological chemistry, 2006 - ASBMB
The small family of G-protein-coupled receptor kinases (GRKs) regulate cell signaling by
phosphorylating heptahelical receptors, thereby promoting receptor interaction with β-
arrestins. This switches a receptor from G-protein activation to G-protein desensitization,
receptor internalization, and β-arrestin-dependent signal activation. However, the specificity
of GRKs for recruiting β-arrestins to specific receptors has not been elucidated. Here we use
the β 2-adrenergic receptor (β 2 AR), the archetypal nonvisual heptahelical receptor, as a …
phosphorylating heptahelical receptors, thereby promoting receptor interaction with β-
arrestins. This switches a receptor from G-protein activation to G-protein desensitization,
receptor internalization, and β-arrestin-dependent signal activation. However, the specificity
of GRKs for recruiting β-arrestins to specific receptors has not been elucidated. Here we use
the β 2-adrenergic receptor (β 2 AR), the archetypal nonvisual heptahelical receptor, as a …