[HTML][HTML] In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death
Gene targeting experiments have demonstrated that the expression of immunoglobulin
heavy chain in the pre-B cell receptor (pBCR) and of heavy and light chains in the B cell
antigen receptor (BCR) marks checkpoints in early B cell development that the cells have to
pass to survive. To investigate whether the persistence of mature B cells in the peripheral
immune system also depends on BCR expression, we have generated a transgenic mouse
in which the BCR can be inducibly ablated through V region gene deletion. Ablation leads to …
heavy chain in the pre-B cell receptor (pBCR) and of heavy and light chains in the B cell
antigen receptor (BCR) marks checkpoints in early B cell development that the cells have to
pass to survive. To investigate whether the persistence of mature B cells in the peripheral
immune system also depends on BCR expression, we have generated a transgenic mouse
in which the BCR can be inducibly ablated through V region gene deletion. Ablation leads to …
Abstract
Gene targeting experiments have demonstrated that the expression of immunoglobulin heavy chain in the pre-B cell receptor (pBCR) and of heavy and light chains in the B cell antigen receptor (BCR) marks checkpoints in early B cell development that the cells have to pass to survive. To investigate whether the persistence of mature B cells in the peripheral immune system also depends on BCR expression, we have generated a transgenic mouse in which the BCR can be inducibly ablated through V region gene deletion. Ablation leads to rapid death of mature B lymphocytes, which is preceded by down-regulation of MHC antigens and up-regulation of CD95 (Fas) and can be delayed by constitutive bcl-2 expression.
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