Lentiviral vector delivery of recombinant small interfering RNA expression cassettes
MJ Li, JJ Rossi - Methods in enzymology, 2005 - Elsevier
Lentiviral vectors are able to transduce nondividing cells and maintain sustained long-term
expression of transgenes. Many cells types, including brain, liver, muscle and hematopoietic
stem cells, have been successfully transduced with lentiviral vectors carrying a variety of
genes. These properties make lentiviral vectors attractive vehicles for delivering small
interfering RNA (siRNA) genes into mammalian cells. RNA polymerase III (pol III) promoters
are most commonly used for expressing siRNAs from lentiviral vectors. Pol III promoters are …
expression of transgenes. Many cells types, including brain, liver, muscle and hematopoietic
stem cells, have been successfully transduced with lentiviral vectors carrying a variety of
genes. These properties make lentiviral vectors attractive vehicles for delivering small
interfering RNA (siRNA) genes into mammalian cells. RNA polymerase III (pol III) promoters
are most commonly used for expressing siRNAs from lentiviral vectors. Pol III promoters are …