Generation of hydrogen peroxide in cultured porcine thyroid cells: synergistic regulation by cytoplasmic free calcium and protein kinase C

N Takasu, T Yamada, Y Shimizu… - Journal of …, 1989 - joe.bioscientifica.com
N Takasu, T Yamada, Y Shimizu, Y Nagasawa, I Komiya
Journal of endocrinology, 1989•joe.bioscientifica.com
This study set out to elucidate the mechanism by which H 2 O 2 generation is regulated in
cultured porcine thyroid cells. We monitored continuously the effects of the calcium
ionophore A23187 and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on H
2 O 2 generation, using homovanillic acid and horseradish peroxidase. A23187 and TPA
stimulated H 2 O 2 generation. A23187 increased cytoplasmic free calcium and TPA
activated protein kinase C. Generation of H 2 O 2 is therefore regulated by cytoplasmic free …
Abstract
This study set out to elucidate the mechanism by which H 2 O 2 generation is regulated in cultured porcine thyroid cells. We monitored continuously the effects of the calcium ionophore A23187 and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on H 2 O 2 generation, using homovanillic acid and horseradish peroxidase. A23187 and TPA stimulated H 2 O 2 generation. A23187 increased cytoplasmic free calcium and TPA activated protein kinase C. Generation of H 2 O 2 is therefore regulated by cytoplasmic free calcium and protein kinase C. Exposure to A23187 or TPA augmented further the stimulation of H 2 O 2 generation by TPA or A23187 respectively. Thus A23187 and TPA, by increasing cytoplasmic free calcium and activating protein kinase C respectively, synergistically activate H 2 O 2 generation.
Journal of Endocrinology (1989) 120, 503–508
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