Glutamine deprivation causes enhanced plating efficiency of a herpes simplex virus type 1 ICP0-null mutant
RM Bringhurst, AA Dominguez, PA Schaffer - Journal of virology, 2008 - Am Soc Microbiol
RM Bringhurst, AA Dominguez, PA Schaffer
Journal of virology, 2008•Am Soc MicrobiolIsoleucine deprivation of cellular monolayers prior to infection has been reported to result in
partial complementation of a herpes simplex virus type 1 (HSV-1) ICP0 null (ICP0−) mutant.
We now report that glutamine deprivation alone is able to enhance the plating efficiency of
an ICP0− virus and that isoleucine deprivation has little or no effect. Because a low
glutamine level is associated with stress and because stress is known to induce reactivation,
low levels of glutamine may be relevant to the reactivation of HSV-1 from latency …
partial complementation of a herpes simplex virus type 1 (HSV-1) ICP0 null (ICP0−) mutant.
We now report that glutamine deprivation alone is able to enhance the plating efficiency of
an ICP0− virus and that isoleucine deprivation has little or no effect. Because a low
glutamine level is associated with stress and because stress is known to induce reactivation,
low levels of glutamine may be relevant to the reactivation of HSV-1 from latency …
Abstract
Isoleucine deprivation of cellular monolayers prior to infection has been reported to result in partial complementation of a herpes simplex virus type 1 (HSV-1) ICP0 null (ICP0−) mutant. We now report that glutamine deprivation alone is able to enhance the plating efficiency of an ICP0− virus and that isoleucine deprivation has little or no effect. Because a low glutamine level is associated with stress and because stress is known to induce reactivation, low levels of glutamine may be relevant to the reactivation of HSV-1 from latency. Additionally, we demonstrate that arginine and methionine deprivation result in partial complementation of the ICP0− virus.
American Society for Microbiology