Targeted integration into a rRNA locus results in uniform and high level expression of transgenes in Leishmania amastigotes
A Mißlitz, JC Mottram, P Overath, T Aebischer - Molecular and biochemical …, 2000 - Elsevier
This report describes the construction of a DNA cassette for integration into a genomic small
sub-unit rRNA locus of Leishmania mexicana by homologous recombination. Reporter
genes encoding β-galactosidase or green fluorescent protein and the gene conferring
hygromycin resistance were integrated downstream of a RNA polymerase I-driven rRNA
promoter. To ensure high expression of the marker proteins in the intracellular, amastigote
stage, transgene coding sequences were followed by the intergenic region of the L …
sub-unit rRNA locus of Leishmania mexicana by homologous recombination. Reporter
genes encoding β-galactosidase or green fluorescent protein and the gene conferring
hygromycin resistance were integrated downstream of a RNA polymerase I-driven rRNA
promoter. To ensure high expression of the marker proteins in the intracellular, amastigote
stage, transgene coding sequences were followed by the intergenic region of the L …