Clinical evaluation of a modified ELISA, using photobiotinylated DNA, for the detection of anti-DNA antibodies
MN Hylkema, H Huygen, C Kramers, TJ Wal… - Journal of …, 1994 - Elsevier
The measurement of anti-dsDNA antibodies is important for the diagnosis and the follow-up
of patients with systemic lupus erythematosus (SLE). For routine detection of anti-dsDNA,
the Farr assay and the immunofluorescence technique (IFT) on Crithidia luciliae proved to
be very useful. The anti-dsDNA ELISA is not used for routine purposes in our institute since it
is flawed by false-positive results due to binding of negatively charged (immune) complexes
to the employed precoat (protamine sulphate). Recently, a new anti-dsDNA ELISA has been …
of patients with systemic lupus erythematosus (SLE). For routine detection of anti-dsDNA,
the Farr assay and the immunofluorescence technique (IFT) on Crithidia luciliae proved to
be very useful. The anti-dsDNA ELISA is not used for routine purposes in our institute since it
is flawed by false-positive results due to binding of negatively charged (immune) complexes
to the employed precoat (protamine sulphate). Recently, a new anti-dsDNA ELISA has been …