[HTML][HTML] Granulation Rescue and Developmental Marking of Juxtaglomerular Cells Using “Piggy-BAC” Recombination of the Mouse RenLocus
LJ Mullins, CM Payne, N Kotelevtseva… - Journal of Biological …, 2000 - ASBMB
Mice lacking a functionalRen-1 d gene exhibit a complete lack of renal juxtaglomerular cell
granulation and atypical macula densa morphology. Transgenic mice carrying a 145-
kilobase BAC clone encompassing the Ren-1 d and Ren-2 loci were generated,
characterized, and backcrossed withRen-1 d−/− mice. HomozygousRen-1 d-null mice
expressing the BAC clone exhibited complete restoration of normal renal structure.
Homologous recombination in Escherichia coli was used to generate a modified version of …
granulation and atypical macula densa morphology. Transgenic mice carrying a 145-
kilobase BAC clone encompassing the Ren-1 d and Ren-2 loci were generated,
characterized, and backcrossed withRen-1 d−/− mice. HomozygousRen-1 d-null mice
expressing the BAC clone exhibited complete restoration of normal renal structure.
Homologous recombination in Escherichia coli was used to generate a modified version of …