[HTML][HTML] Mammalian wax biosynthesis: I. Identification of two fatty acyl-coenzyme A reductases with different substrate specificities and tissue distributions
JB Cheng, DW Russell - Journal of biological Chemistry, 2004 - ASBMB
The conversion of fatty acids to fatty alcohols is required for the synthesis of wax monoesters
and ether lipids. The mammalian enzymes that synthesize fatty alcohols have not been
identified. Here, an in silico approach was used to discern two putative reductase enzymes
designated FAR1 and FAR2. Expression studies in intact cells showed that FAR1 and FAR2
cDNAs encoded isozymes that reduced fatty acids to fatty alcohols. Fatty acyl-CoA esters
were the substrate of FAR1, and the enzyme required NADPH as a cofactor. FAR1 preferred …
and ether lipids. The mammalian enzymes that synthesize fatty alcohols have not been
identified. Here, an in silico approach was used to discern two putative reductase enzymes
designated FAR1 and FAR2. Expression studies in intact cells showed that FAR1 and FAR2
cDNAs encoded isozymes that reduced fatty acids to fatty alcohols. Fatty acyl-CoA esters
were the substrate of FAR1, and the enzyme required NADPH as a cofactor. FAR1 preferred …
