Transplantation of intact pancreatic islets in rats
WF Ballinger, PE Lacy - Surgery, 1972 - surgjournal.com
WF Ballinger, PE Lacy
Surgery, 1972•surgjournal.com1. A method of isolation of pancreatic islets in the rat is described. These islets respond by
increasing insulin production when perifused with high concentrations of glucose. 2.2.
Histologic examination of the pancreas of recipient rats confirms the marked beta cytotoxic
effect of streptozotocin. 3.3. The transplantation of approximately 400 to 600 pancreatic islets
to the peritoneal cavity or to the thigh muscle in inbred diabetic Lewis rats results in a
significant reduction of hyperglycemia, polyuria, and glycosuria and a restoration of weight …
increasing insulin production when perifused with high concentrations of glucose. 2.2.
Histologic examination of the pancreas of recipient rats confirms the marked beta cytotoxic
effect of streptozotocin. 3.3. The transplantation of approximately 400 to 600 pancreatic islets
to the peritoneal cavity or to the thigh muscle in inbred diabetic Lewis rats results in a
significant reduction of hyperglycemia, polyuria, and glycosuria and a restoration of weight …
Abstract
- 1.1. A method of isolation of pancreatic islets in the rat is described. These islets respond by increasing insulin production when perifused with high concentrations of glucose.
- 2.2. Histologic examination of the pancreas of recipient rats confirms the marked beta cytotoxic effect of streptozotocin.
- 3.3. The transplantation of approximately 400 to 600 pancreatic islets to the peritoneal cavity or to the thigh muscle in inbred diabetic Lewis rats results in a significant reduction of hyperglycemia, polyuria, and glycosuria and a restoration of weight gain.
- 4.4. Excision of transplanted islets from muscle results in return to the fully diabetic state. Histologic examination of the excised islets reveals intact beta cells, with degranulation, indicating the great demand for insulin on these cells.
- 5.5. Allografts of pancreatic islets in diabetic immunosuppressed rats also function sufficiently to reduce partially the severe diabetic state.
- 6.6. Human pancreatic islets have been isolated and produce insulin ex vivo in a perifusion chamber when challenged with high concentrations of glucose.
- 7.7. It is suggested that transplantation of pancreatic islets, isolated from acinar and ductal pancreatic tissue, may be preferable to whole organ pancreaticoduodenal transplantation.
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